Date of Birth: August 13, 1986, Marital status: Married Citizenship: Indian
West Bengal Board of Secondary Education (W.B.B.S.E)
St. Johns’ Diocesan Girls H.S. School
West Bengal Council of Secondary Education (W.B.C.H.S.E)
National High School for Girls
B.Sc. (Zoology Hons.)
University of Calcutta
Jogamaya Devi College
68* *Chaya Ghosh Memorial Award from college for scoringhighest in Zoology
University of Calcutta
Ballygunge Science College
Ph.D. (Biotechnology and Immunology)
National Institute of Cholera and Enteric Diseases (Division of Immunology)
SERB National Post-doctoral fellow
Department of Biotechnology
National Centre for Cell Science
September 2016- January 2017
Other Courses Taken
Two Credit Course on Genomics and Biotechnology by MHRD and University of Pune
Bio-informatics tools and application
Institutional Biosafety Training
Recipient of SERB National Post-doctoral fellowship and functioned as Principal Investigator of the project
Qualified CSIR/UGC NET December 2010 examination as JRF
GATE 2011 qualified with 94 percentile.
10th In M.Sc (Zoology) University of Calcutta.
1st B.Sc In College (C.U).
Sinha, D., Ghosh, A. K., Mukherjee, S., Biswas R. and Biswas. T. 2015. Porin differentiates TLR mediated proinflammatory response of follicular zone B cell from TLR-unresponsive IL-10 expressing marginal zone B cell. 76: 193-205.
Ghosh, A. K., Sinha, D., Mukherjee, S., Biswas R. and Biswas. T. 2015. LPS stimulates and Hsp70 down-regulates TLR4 to orchestrate differential cytokine response of culture-differentiated innate memory CD8+ T cells. Cytokine 73: 44-52. (Joint First Author).
Sinha, D., Ghosh, A. K., Mukherjee, S., Biswas, R. and Biswas, T. (2014) Antigenic relatedness defines Toll-like receptor 2 is crafted on ligand blueprint. Immunobiology 219: 798-801.
Ghosh, A. K., Sinha, D., Mukherjee, S., Biswas, R. and Biswas, T. (2015) IL-15 temporally reorients IL-10 biased B-1a cells toward IL-12 expression. Cell. Mol. Immunol. 13: 229-239.
Mukherjee, S., Sinha, D., Ghosh, A. K. and Biswas, T. (2014) Bacterial ligand stimulates TLR2-dependent chemokines of colon cell. Immunobiology 219: 350-356.
Biswas, R., Mukherjee, S., Sinha, D., Ghosh, A. K. and Biswas, T. (2014) Culture-differentiated CD8+ T cells acquire innate memory-like traits and respond to a pathogen-associated molecule. Immunol. Cell Biol. 92: 368-376.
Ghosh, A. K., Sinha, D., Biswas, R. and Biswas, T. (2016) Pathogen-associated porin turns IL-10 competent B-1a cells toward proinflammatory cytokine response. Immunobiology. 221: 1369-1373.
Ghosh, A. K., Sinha, D., Biswas, R. and Biswas, T. (2017) IL-15 stimulates NKG2D while promoting IgM expression of B-1a cells. Cytokine 95: 43-50.
Total five years of experience in handling and operation of BD FACSAria II (Three laser 9 colour system) and BD FACSCalibur (Two laser 4 colour system) along with their operating soft wares FACSDiva and CellQuest respectively. Hands-on Experience on the Following Applications of Flow Cytometer:
Cell sorting after staining with flurochrome conjugated antibodies of surface markers (Purification of Follicular an Marginal Zone B cells from spleen, CD4+ T cell from thymus, and B-1a cell from peritoneal cavity of mouse)
Immunophenotyping of both primary cells and secondary cell lines.
Intracellular cytokine assay by blocking cytokines in Golgi body and then its detection by respective flurochrome conjugated monoclonal antibodies.
Cell proliferation assay using CellTrace™ CFSE Cell Proliferation Kit
Apoptosis analysis by Annexin V vs PI staining
TUNNEL Assay with Apo-Direct Kit
Cell cycle study by PI staining
Checking of siRNA transfection efficiency in primary cells after conjugating siRNAs with flurochromes.
Magnetic Cell Sorter Operation (Auto MACS)
Purification of bacterial outer membrane protein (porin)
Experimental animal treatment (mouse)
Murine B, T cell and MФ purification and culture
Maintenance and culture of mammalian cell lines
RT-PCR and Real-Time PCR technique
RNA-interference (siRNA knockdown of proteins in murine cell)